Tlc Lab Report - 1670 Words

Separating Substances: Identifying Food Dyes with TLC Background The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summers day or a golden Thanksgiving turkey garnished with fresh green parsley? Even early civilizations such as the Romans recognized that people eat with their eyes as well as their palates. Saffron and other spices were often used to provide a rich yellow color to various foods. Butter has been colored yellow as far back as the 1300s. Food color additives are dyes, pigments or substances that impart color when applied to a food, drug, cosmetic, or the human body. The Food and Drug Administration†¦show more content†¦Place 5 candy pieces of the same color into a small beaker. Pour enough acetic acid solution into each tube to just cover all of the candy pieces, around 5 mL. 2. Agitate the beaker carefully for about 30-45 seconds, or until the color comes off of the candy. Be careful to dissolve as little as possible of the white layer (sugar) under the colored layer and do not dissolve any of the chocolate. Immediately decant the colored solution into a clean test tube, trying not to transfer any of the sediment. Repeat with the other colors for which you are responsible. 3. Now you need to extract the dye from the solution onto the strips of yarn. To do this, place one piece of yarn into each test tube of colored solution. Heat the tubes in a boiling water bath for 8-10 minutes. You can remove the tubes from the water when the solution is milky-white and the yarn is the color of the dye, in other words, when all of the dye has been extracted from the solution. 4. Remove the dyed yarn from the solution and rinse it with distilled water. If you wish you may cut ~2 cms off each piece of yarn as a souvenir. Let these dry on a piece of paper, and you may take them away with you to keep ifShow MoreRelatedLab Report on TLC analisys of Analgestic Drugs Essay examples1266 Words   |  6 PagesTLC Analysis of Analgesic Drugs Introduction In this experiment, thin-layer chromatography (TLC) was used to determine the composition of various over-the-counter (OTC) analgesics: Anacin, Bufferin, Excedrin, and Tylenol. The TLC plates were first viewed under ultraviolet (UV) light and then treated with iodine vapor in order to visualize the spotting. Experiment Scheme Initially, sixteen capillary micropipets were created in order to spot the TLC plates. Two TLC plates were then obtainedRead MoreLab Report On Chromatography And Lab1206 Words   |  5 PagesChromatography and TLC of Spinach Group Experiment 3 – Individual Lab Report (Save as pdf and submit, due by 12:00 NOON one week after experiment) Last Name: Wang First Name: Zoey Lab Partner(s): Sue Wang TA Name: Sosthene Date Lab Performed: Nov. 12, 2015 Date Lab Submitted: Nov. 12, 2015 Group A, B, or C: B Comments for Grading TA: (Please indicate if you performed the lab on a day other than your regularly scheduled day and/or with a TA other than your regular TA). Page Limit: report must not exceedRead MoreExperiment 2a Adsorption Chromatography ( Tlc )1455 Words   |  6 PagesNAME: ____Amy Hua_______________________ Experiment 4a Adsorption Chromatography (TLC) Summary of Points for Experiment 4a: Item Possible Points Actual Points Pre-Lab 2 Notebook: N/A N/A Purpose/Table of Reagents 2 Corrections 2 Blank Spaces 2 Signatures 2 TLC data (4-in notebook) 8 Coherent 2 Conclusions (absent here) 1 Sub-Total = 21 multiply Sub-Total x 2= 42 Report: N/A N/A Introduction 2 Data and Calculations 8 Less Points-Missing Data N/A N/A Unknown Identity 10 Read MoreChemistry Experiment : Oxidation Of Borneol1607 Words   |  7 Pages Experiment 3: Oxidation of Borneol to Camphor By: Evan Holley Lab partner: Jeffrey Horton T.A.: Garret CHM2211L-008 07/12/2015 Introduction The overall goal in this lab was to oxidize borneol, a secondary alcohol, into camphor, which is a ketone. For the purposes of oxidation chromic acid was utilized, which was prepared by adding a 1:1 ratio of chromium trioxide to dilute sulfuric acid. The oxidation reaction occurs as a two-step reaction. The first step involves the formationRead MoreOrganic Chem 1 Post Lab Report Essay848 Words   |  4 PagesPost Lab Report Experiment 3 - Chromatography – Analyzing Analgesics by TLC and Isolation of ÃŽ ²Ã¢â‚¬â€œCarotene by Column Chromatography Chemicals 1. Acetaminophen (C8H9NO2) 2. Aspirin (C9H8O4) 3. Caffeine (C8H10N4O2) 4. Ibuprofen (C13hH18O2) Introduction In this experiment, several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions byRead MoreEssay about Organic Chemistry Ii Lab Report1715 Words   |  7 PagesTorres 1 Luis A. Torres Group #11 USC Chemistry 322b Formal Lab Report 6th November 2015 I. II. Enzymatic Resolution of 1-Phenylethanol and Diastereomer Analysis Objective/Abstract Enzymatic transesterification reaction was performed to study the resolution of diastereomers using 1H-NMR analysis. The stereo-selectivity of acylase I, an enzyme, for a 50:50 racemic mixture of 1-phenylethanol was determined. In the first of a two-step reaction, 1-phenylethanol was reacted with vinylRead MoreIdentification of Unknown Ketones Essay1152 Words   |  5 Pages811 | 106 | 2-heptanone | 114.2 | 151 | .811 | 89 | 2-octanone | 128.2 | 173 | .819 | 58 | Reaction/Calculations: There were no reactions in this lab. Known ketones: 2-propanone 2-butanone 2-hexanone 2-heptanone 2-octanone Mass of unknown: .17g Melting Point of Unknown: 87 deg C TLC Slides: Rf Values: Unknown K = 3cm/6cm = .5 2-Propanone: 2.6cm/5.5cm = .47 2-Hexanone: 2.9cm/5.4cm = .53 2-Heptanone: 3.2cm/6.2cm = .51 Read MoreWhodunit: A Forensic Investigation1620 Words   |  7 PagesPike Date submitted: 9/26/2012 Lab Partner: Charles Stewart CHM1046L – sec#21 Purpose: The purpose of this experiment was to investigate a crime scene. Given an evidence bag a number of tests were performed in order to determine who the suspect was. The experiments included a simple fiber burn test, iodine fuming to develop a finger print, and thin-layer chromatography to determine ink similarities. Procedure: No changes were made to the procedure in the lab manual. Dillon, Stephanie. WhodunitRead MoreSynthesis and Characterization of Aspirin Product Identity and Purity After synthesizing a1000 Words   |  4 Pagespure acetylsalicylic acid. 5. Place the sealed end of the two tubes into the melting point apparatus as follows: your product on the left and pure acetylsalicylic acid on the right. You may need to share a melting point apparatus with another two lab partners. In this case, since there are only three sample slots in the apparatus, put the pure acetylsalicylic acid in the middle and use the two outside slots for the synthesized products. 6. Heat the apparatus rapidly to about 80 °C, then slowly heatRead MoreEssay about spirometry report953 Words   |  4 PagesSpirometry Lab Report: Name__________ Lab Section: 1 2 3 Lab Partner(s) _________________ Use your PowerPoint notes, lab book, text book, your data, and the Internet to answer the following questions. All reports must be typed and stapled. All reports must be in your own words. One student from each group should include the LabTutor-generated data tables and/or graphs. Exercise 1: Pulmonary